Objectives: The aim of this study was to examine the effects of SIRT1 on EAE severity using a transgenic SIRT1 overexpressing mouse.
Methods: EAE was induced in wild type and SIRT1 overexpressing C57Bl/6J mice with oligodendroglial glycoprotein peptide (MOG35-55) using previously reported methods (ref above). Mice were examined, scored clinically and brains and spinal cords examined immunohistochemically.
Results: Clinical severity was reduced in the overexpressing mice compared to wild type and this correlated with reduced demyelination, less axonal loss and a reduction in inflammatory infiltration. Fewer apoptotic cells were found in the tissue of SIRT1 overexpressing mice.
Conclusions: These results are consistent with a neuroprotective effect of SIRT1 in EAE but further studies will be required to determine whether the reduction in severity is an indirect effect of SIRT1 in the overexpressing mouse.