Cuprizone Mouse Model of Demyelination: Environmental Enhancement Reverses Cognitive Deficits
Impairment in cognitive functioning and motor activity are commonly encountered in patients affected by multiple sclerosis (MS).
To investigate the effect of enhanced environment on cuprizone mouse model of demyelination.
C57BL/6 male mice were divided into cuprizone only (Cup-O), cuprizone housed in EE (Cup-EE) and control groups (9 to 10 per group). Environmental enhancement continued for a period of nine weeks. Neurobehavioral tests were conducted after a six-week period of 0.2% cuprizone-enriched diet. All mice were assessed for cognitive performance in the Morris water maze, motor function in rotarod and exploratory behavior in open field test.
Cup-EE performed significantly better in fields of spatial learning and memory and motor functioning when compared to Cup-O as evident by Morris water maze (p<0.001) and rotarod (p<0.05) results. Open field test results failed to show an anxiety-like behavior in cuprizone mouse model.
Environmental enhancement can improve MS-associated cognitive and motor deficits. Insights gained from these results facilitate the exploration of non-medical modes of intervention as an emerging adjuvant therapy in MS.